| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moch, Hope M (10)
|
NITTAC |
27.52 |
23.32 |
-4.20 |
| 2 |
Canaple, Isabelle A (10)
|
NITTAC |
30.00 |
23.52 |
-6.48 |
| 3 |
Neuhaus, Claire K (8)
|
NIGISC |
26.29 |
24.84 |
-1.45 |
| 4 |
Yasurek, Julianna J (10)
|
NIPVSC |
37.50 |
26.08 |
-11.42 |
| 5 |
Sellan, Grace I (8)
|
NITTAC |
26.11 |
26.44 |
0.33 |
| 6 |
Hosmer, Sadie M (9)
|
NIPVSC |
35.19 |
26.71 |
-8.48 |
| 7 |
Dundon, Jordyn M (8)
|
NITTAC |
27.74 |
26.81 |
-0.93 |
| 8 |
Stockinger, Audrey M (9)
|
NIGISC |
28.00 |
26.88 |
-1.12 |
| 9 |
Moch, Faith L (10)
|
NITTAC |
28.64 |
27.66 |
-0.98 |
| 10 |
Bronson, Maxwell F (10)
|
NIGISC |
35.89 |
31.13 |
-4.76 |
| 11 |
Jayme, Nicholas D (8)
|
NIGISC |
|
37.37 |
- |
| 12 |
Sunick, Trent S (8)
|
NIPVSC |
|
37.66 |
- |
|
Avalos, Tyler J (8)
|
NITTAC |
37.00 |
DQ |
- |
|
Vandriel, Annabelle k (8)
|
NIGISC |
|
NS |
- |